col1a2 (Proteintech)
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Proteintech
col1a2
Col1a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+col1a2/Collagen+Type+I+Polyclonal+antibody/pm41850661-127-19-20
Average 96 stars, based on 992 article reviews
Col1a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+col1a2/Collagen+Type+I+Polyclonal+antibody/pm41850661-127-19-20
Average 96 stars, based on 992 article reviews
col1a2 - by Bioz Stars,
2026-10
96/100 stars
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Incubation:Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 °C overnight ( Article Title: Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection Article Snippet: Lysates containing equal amounts of total protein were subjected to 6%–12% sodium dodecyl sulfate–PAGE (SDS–PAGE) and transferred onto polyvinylidene fluoride membranes (Millipore, Burlington, MA, USA). .. The membranes were blocked using a blocking reagent [Tris-buffered saline with 5% ( w / v ) non-fat dry milk and 0.1% ( v / v ) Tween 20, pH 7.4] for 1 h at room temperature and then incubated with the following primary antibodies at 4 °C overnight: anti-NFATc3 (#4998; 1:1000), anti-lamin B1 (#13435; 1:1000), anti-phospho-eEF2 (Thr56) (#2331; 1:1000), anti-eEF2 (#2332; 1:1000), eEF2k (#3692; 1:1000) (Cell Signaling Technology), anti-LDL-R (sc-11824; 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-MMP2 (ab97779; 1:500), anti-MMP9 (ab76003; 1:1000), anti-RPS6 (ab225676; 1:1000), anti-RPL28 (ab254927; 1:500), anti-protein phosphatase 2A (PP2A; ab32065; 1:500), anti-Flag (ab205606; 1:500), anti-his (ab213204; 1:5000) (Abcam), anti-fibronectin (15613-1-AP; 1:2000), Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging. Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 ◦C overnight ( Article Title: Complement protein C1q induces endothelial apoptosis through C1qBP regulation of mitochondrial function in lung endothelial cells in systemic sclerosis. Article Snippet: .. Following addition of endogenous peroxidase blocker, the sections were incubated overnight at 4°C with the following primary antibodies: anti-C1qBP (1:100, MA1-40312, Thermo fisher), anti-α-SMA AR TIC LE IN PR ES S (1:100, BM3902, Boster), Saline:Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 °C overnight ( Article Title: Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection Article Snippet: Lysates containing equal amounts of total protein were subjected to 6%–12% sodium dodecyl sulfate–PAGE (SDS–PAGE) and transferred onto polyvinylidene fluoride membranes (Millipore, Burlington, MA, USA). .. The membranes were blocked using a blocking reagent [Tris-buffered saline with 5% ( w / v ) non-fat dry milk and 0.1% ( v / v ) Tween 20, pH 7.4] for 1 h at room temperature and then incubated with the following primary antibodies at 4 °C overnight: anti-NFATc3 (#4998; 1:1000), anti-lamin B1 (#13435; 1:1000), anti-phospho-eEF2 (Thr56) (#2331; 1:1000), anti-eEF2 (#2332; 1:1000), eEF2k (#3692; 1:1000) (Cell Signaling Technology), anti-LDL-R (sc-11824; 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-MMP2 (ab97779; 1:500), anti-MMP9 (ab76003; 1:1000), anti-RPS6 (ab225676; 1:1000), anti-RPL28 (ab254927; 1:500), anti-protein phosphatase 2A (PP2A; ab32065; 1:500), anti-Flag (ab205606; 1:500), anti-his (ab213204; 1:5000) (Abcam), anti-fibronectin (15613-1-AP; 1:2000), Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging. Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 ◦C overnight ( Blocking Assay:Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 °C overnight ( Article Title: Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection Article Snippet: Lysates containing equal amounts of total protein were subjected to 6%–12% sodium dodecyl sulfate–PAGE (SDS–PAGE) and transferred onto polyvinylidene fluoride membranes (Millipore, Burlington, MA, USA). .. The membranes were blocked using a blocking reagent [Tris-buffered saline with 5% ( w / v ) non-fat dry milk and 0.1% ( v / v ) Tween 20, pH 7.4] for 1 h at room temperature and then incubated with the following primary antibodies at 4 °C overnight: anti-NFATc3 (#4998; 1:1000), anti-lamin B1 (#13435; 1:1000), anti-phospho-eEF2 (Thr56) (#2331; 1:1000), anti-eEF2 (#2332; 1:1000), eEF2k (#3692; 1:1000) (Cell Signaling Technology), anti-LDL-R (sc-11824; 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-MMP2 (ab97779; 1:500), anti-MMP9 (ab76003; 1:1000), anti-RPS6 (ab225676; 1:1000), anti-RPL28 (ab254927; 1:500), anti-protein phosphatase 2A (PP2A; ab32065; 1:500), anti-Flag (ab205606; 1:500), anti-his (ab213204; 1:5000) (Abcam), anti-fibronectin (15613-1-AP; 1:2000), Article Title: Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging. Article Snippet: Proteins were electrophoretically transferred to 0.2 μm nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. Membranes were incubated with 5% milk in Tris-Buffered Saline with 0.1% Tween 20 (TBS-T) to block non-specific sites and then with primary antibodies in 5% milk in TBS-T at 4 ◦C overnight ( Article Title: Vitamin D 3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells. Article Snippet: .. After washing and blocking with TBS-T and 2.5% non-fat milk respectively, the membranes were separately immunoblotted overnight at 4 ◦C with rabbit polyclonal anti-TGFB1i1 (Cat No: 10565-1-AP), anti-α-SMA (Cat No: 14395-1-AP), |
